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Contents of README:PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR) and sequencing. It aims to automate and simplify the process of primer design. PerlPrimer's current features include the following: - Calculation of possible primer-dimers - Retrieval of genomic or cDNA sequences from Ensembl (including both sequences automatically for QPCR) - Ability to BLAST search primers using the NCBI server or a local server - Results can be saved or optionally exported in a tab-delimited format that is compatible with most spreadsheet applications. - ORF and CpG island detection algorithms - Ability to add cloning sequences to primers, automatically adjusted to be in-frame - QPCR primer design without manual intron-exon boundary entry An optional run-time dependency is spidey, for Real Time PCR. For details and citation: Marshall OJ. PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR. Bioinformatics 2004 20(15):2471-2472 |
Name Last modified Size
Parent Directory - README 04-Jun-2022 00:08 1.0K References 04-Jun-2022 00:08 158 doinst.sh 04-Jun-2022 00:08 129 perlprimer-f415ea6d0170f2f62f10c678adac8fdc69a3298f.tar.gz 04-Jun-2022 07:39 360K perlprimer.SlackBuild 11-Mar-2022 06:34 2.8K perlprimer.desktop 04-Jun-2022 00:08 126 perlprimer.info 04-Jun-2022 00:08 393 perlprimer.png 05-Sep-2011 00:00 4.8K slack-desc 04-Jun-2022 00:08 936
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